NOC:Animal Transgenesis: Principles and Methods

Lecture 1 - Common terms used in the course

Lecture 2 - Why transgenesis is needed ?

Lecture 3 - Model organisms for transgenesis

Lecture 4 - 3R Repalace Reduce Refine

Lecture 5 - History of transgenesis - I

Lecture 6 - History of transgenesis - II

Lecture 7 - General Principles of Transgenesis in Mouse

Lecture 8 - History of Cell culture

Lecture 9 - Cell culture Types and Basic Requirements

Lecture 10 - Culture medium and Challenges with cell culture

Lecture 11 - Methods for internalization of transgene, and Cell Preservation and Generation of transgene overexpressing mouse

Lecture 12 - Cell for transgenesis and Homologus recombination

Lecture 13 - Targeting vector

Lecture 14 - ES cells, Electroporation, Screening, and Founder or Chimera mice

Lecture 15 - Germline transmission and Conditional knockout mice

Lecture 16 - Example of conditional knockout mice and Temporal control of gene expression

Lecture 17 - Discovery, origin and Types of CRISPR-Cas9 System

Lecture 18 - CRISPR-Cas9 sgRNA and it’s modifications for gene editing

Lecture 19 - CRISPR-Cas9 version and it’s application

Lecture 20 - Genome-wide CRISPR-Cas9 screening and its variants application

Lecture 21 - CRISPR-Cas9 in therapeutics