Lecture 1 - Introduction to Fluorescence Microscopy
Lecture 2 - Introduction to Optics
Lecture 3 - Design of a fluorescence microscope
Lecture 4 - Fluorescent proteins, organic dyes, and protein labelling strategies
Lecture 5 - LIVE Demonstration of the fluorescence microscope, its design and components
Lecture 6 - 6D imaging, live cell imaging, time lapse imaging
Lecture 7 - Quantifying protein dynamics using FRAP, FLIP, FRET
Lecture 8 - LIVE Demonstration for 6D imaging of live cells on a Leica DMi8 inverted fluorescence microscope
Lecture 9 - Types of Illumination Epi, TIRF, HILO, light sheet, two photon
Lecture 10 - Confocal microscopy
Lecture 11 - LIVE Demonstration of the Nikon N-SPARC confocal microscope
Lecture 12 - Super-Resolution Microscopy (SIM, STED, STORM/PALM)
Lecture 13 - Nanomaterials for fluorescence imaging
Lecture 14 - Immunofluorescence IF and Immunohistochemistry IHC
Lecture 15 - Fluorescence In Situ Hybridization FISH, RNA FISH
Lecture 16 - LIVE Demonstration for immunofluroescence procedure
Lecture 17 - Digital images and camera technologies for microscopy
Lecture 18 - CCD, EMCCD, sCMOS Camera
Lecture 19 - LIVE Demonstration for digital images and adjustments
Lecture 20 - Basic operations of ImageJ/FIJI, montage preparation
Lecture 21 - Image filtering and object segmentation
Lecture 22 - Macros, intensity measurement, scale bar, time stamp, colocalization
Lecture 23 - LIVE Demonstration of ImageJ/Fiji based image processing
Lecture 24 - Single molecule imaging and tracking
Lecture 25 - Optical tweezers and traction force microscopy
Lecture 26 - LIVE Demonstration for single-molecule imaging under HILO illumination
Lecture 27 - Spatial transcriptomics and proteomics RNAscope, MERFISH, CODEX
Lecture 28 - High content imaging
Lecture 29 - Designing a fluorescence microscopy experiment
